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sspA [2019-06-25 09:32:03]
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sspA [2019-06-25 09:32:03]

small acid-soluble spore protein (major alpha-type SASP)
Locus
BSU29570
Isoelectric point
4.73
Molecular weight
6.93 kDa
Protein length
69 aa Sequence Blast
Gene length
210 bp Sequence Blast
Function
protection of spore DNA
Product
small acid-soluble spore protein (major alpha-type SASP)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Small acid-soluble spore proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    3,025,445 3,025,654

    Phenotypes of a mutant

  • a sspA mutant is sensitive to ionizing radiation PubMed
  • a sspA sspB double mutant is sensitive to blue light-induced DNA damage PubMed

    • The protein

    Protein family

  • Alpha/beta-type SASP family (according to UniProt)

    • Paralogous protein(s)

  • SspC, SspD, SspB

    • Modification

  • phosphorylated on Ser-6, Ser-9-, Ser-47, and Ser-55 PubMed

    • Structure

  • 2Z3X (SspC in complex with DNA, 83% identity)

    • Expression and Regulation

    Operons

    Genes
    nifZ-thiI-sspA
    Description
    PubMed
    view in new tab

    Genes
    sspA
    Description
    PubMed

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulatory mechanism

  • SpoVT: activation, PubMed, in SpoVT regulon

    • Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
    • view in new tab

    Additional information

  • the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed

    • Biological materials

    Mutant

  • BP137 (sspA::cat) available in Fabian Commichau's lab
  • 1S109 (no resistance), available at BGSC
  • 1S111 (no resistance), PubMed, available at BGSC
  • 1S112 (no resistance), PubMed, available at BGSC
  • 1S113 (no resistance), PubMed, available at BGSC
  • BKE29570 (sspA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT, downstream forward: _UP4_TAATTTACAATTTCACATAA
  • BKK29570 (sspA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT, downstream forward: _UP4_TAATTTACAATTTCACATAA

    • References

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